The Musculoskeletal Physiology Core provides the musculoskeletal science community with phenotyping assays in rodent models.
Christopher W. Ward, PhD
Professor, Department of Orthopaedics
ward@som.umaryland.edu
Joseph P. Stains, PhD
Professor, Department of Orthopaedics, Division Head of Musculoskeletal Science
jstains@som.umaryland.edu
Joella Trenchard
Administrator
jtrenchard@som.umaryland.edu
Hours | Location |
9am-5pm |
Bressler Research Building, 4th Floor, Room 4-038 |
Name | Role | Phone | Location | |
---|---|---|---|---|
Chris Ward, PhD |
Codirector
|
ward@som.umaryland.edu
|
||
Joseph Stains, PhD |
Codirector
|
jstains@som.umaryland.edu
|
||
Joella Trenchard |
Administrator
|
410 706 5937
|
jtrenchard@som.umaryland.edu
|
Service list |
Name | Description | Price |
---|---|---|
Bone Loading |
Application of axial load to bones (current configurations for ulna and tibial loading) to stimulate new bone formation in anesthetized mice. Instrument: Bose electroforce 3200 Time per bone, 20 mins per limb. (consumables) |
Inquire |
ECG Tunnel |
The EMKA ECG Tunnel enables electrocardiogram assessment in the awake, but restrained, mouse. 30 minutes, 2 mice |
Inquire |
Gait Analysis (DigiGait) |
Quantify the gait kinematics as a measure of neuromuscular function in rodents. The DigiGait Gait Analysis System (Mouse Specifics, Inc.; Quincy, MA performs ventral plane videography of mice walking on a transparent treadmill belt. A high-speed, high-resolution digital video camera continuously images the underside of the walking mouse. Software generates digital paw prints and dynamic gait signals, providing a temporal record of paw placement. More than 20 gait indices are reported from the analyses that are related to specific functional gains or losses. (Hour and a half per 10 mice, includes analysis). |
Inquire |
Grip Strength |
An indirect measure of the gross neuromuscular performance of the mouse forelimbs. Effective at identifying dysfunction murine models of motor neuron and muscle disease. 1 min per mouse (minimum charge of half hour - 15 mouse) |
Inquire |
Inverted Grid |
An indirect measure of gross neuromuscular performance of the mouse. Effective at identifying dysfunction murine models of motor neuron and muscle disease. 5 mins per mouse (half hour minimum, 6 mice maximum) |
Inquire |
Mechnical Property Testing |
(1) Investigation of rodent long-bone functional properties by four point bending, including whole-bone structural properties (femoral bending stiffness, maximum moment and failure moment) and bone tissue "material" properties (elastic modulus, yield strength, ultimate strength). (2) Investigation of biomechanical properties of materials under a variety of loading configurations in the axial plane, including custom testing fixtures and testing protocols. Instrument: Bose electroforce 3200 Per bone, 30 mins, 2 bones per hour (minimum 30 minutes) |
Inquire |
Microcomputed Tomography |
Ex vivo quantification of rodent bone trabecular and cortical microarchitecture/morphology and bone tissue mineralization. Additionally, scanning of soft tissues such as heart and cartilage can be done upon special sample preparation and staining with contrast agents. Instrument: Skyscan 1172 microCT. Per hour. (4 hour increments per use)
|
Inquire |
Rotarod |
30 minutes, 5 mice minimum |
Inquire |
Static and Dynamic Histomorphometry |
Analysis software for the quantitation of static and dynamic bone histomorphometry, including osteoblast number, osteoclast number, bone formation rates, mineralizing surface, and mineral apposition rate. Per bone |
Inquire |
Treadmill |
Quantify forced exercise capacity on a motorized treadmill. Most rats and mice are amenable to this test with proper acclimation to the treadmill. We use a 6 lane rodent treadmill (Columbus Instruments). per hour, up to 6 mice |
Inquire |