How to acknowledge us in your publications? University of Maryland School of Medicine’s Viral Vector Core – Baltimore, Maryland.
MISSION
UMB Virus Vector Core (VVC) offers Adeno Associated Virus (AAV) and lentivirus (LV)-based gene delivery systems to be used in gene therapy for targeting a gene of interest (GOI) in in-intro and in-vivo studies. The VVC offers services for custom gene cloning and packaging in AAV or LV. The mission of this Core is to provide high-quality viral vectors, reducing the time and cost for the investigators utilizing modern tools and technology. The Core offers free consultancy for gene construct designing strategy, discussing the research project, implementation, and troubleshooting.
SERVICES OFFERED
AAV virus
AAV virus is packaged by using triple transfection in HEK393T cells. Helper plasmid, red/cap plasmid, and transgene plasmid. After 72hr of transfection, the virus is purified by ultracentrifugation method.
Titer- We are routinely providing a very high titer range from 1012 to 1013 GC/ml, Note- titer may depend on the loading size of the target gene and serotype.
Quality control
Lentivirus
UMB Viral Vector Core generates the lentivirus using a second-generation lentivirus system. We are using standard psPAX2 and envelop protein CMV-SVS-G/ pMD.2G plasmid transfected into HEK293 cell line. Lentivirus containing clear supernatant concentrated by ultracentrifugation. Our service provides in-vivo and in-vitro grade ultra-purified lentivirus.
Lenti titer- We used Lenti-X™ qRT-PCR Titration Kit (TAKARA) or used a standard method to determine the LV concentration.
Service Amount/Titer Internal Price External Price Corporate price
Lentivirus
Lentiviral test sample (supernatant for cell culture). 10ml, 107 – 108 TU/ml $255.00 $315.00
Ultra-purified in-vivo grade concentrated Lentivirus (small scale) 500µl, 108–109 TU/ml $1215.00 $1315.00 $1550
Ultra-purified in-vivo grade concentrated Lentivirus (large scale) 1000µl, 108–109TU/ml $1450.00 $1550.00 $1950
AAV virus
AAV Customize virus packaging 700-1ml, 1012–1013GC/ml $1950.00 $2350.00 $2550
AAV stock virus 100µl,1012–1013 GC/ml $320.00 $350.00 $380.0
NOTE-
1-All 'internal' and 'external' users please sign in iLab system to pay the bill.
2-All the above cost subjects are to update every financial year on July 1.
3-Please deposite/ship the samples at 655 West Baltimore Street, BRB Rm7018, Baltimore, 21201
Ramesh Chandra | Director | 410 706 2770 | rchandra@som.umaryland.edu |
Name | Role | Phone | Location | |
---|---|---|---|---|
Ramesh Chandra, PhD |
Director
|
410 706 2770
|
RChandra@som.umaryland.edu
|
655 West Baltimore Street, BRB Rm7018, Baltimore,21201
|
Service list |
► DNA Prep (1) | |||
Name | Description | Price | |
---|---|---|---|
Bacteria glycerol stock |
Bacteria glycerol stocks will be in sterile 50% glycerol in cryogenic tubes. |
Inquire |