Translational Laboratory Shared Service (UMGCC)

Overview of Services

The University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) Translational Shared Service (TLSS) offers pre-clinical and clinical experimental support to basic researchers and physicians in the UMGCC community. We work in areas across the entire spectrum: cell biology, in vitro, in vivo and human trials. 

 

In Vitro Assays 

  • IC50 generation
  • Cell cycle (propidium iodide)
  • Viability (trypan blue exclusion)
  • Apoptosis
  • DNA Damage (Gamma-H2Ax Immunofluorescence)
  • Potentiation/Synergy
  • ROS
  • Western Analysis
  • Telomere Length Assay (QPCR)
  • Angiogenesis
  • Mycoplasma testing

 

Xcelligence 

  • Real time proliferation/invasion/migration

 

In Vivo Assays 

  • IACUC approved umbrella protocol
  • Tolerability:
  • Tumor Growth
  • Pharmacokinetics: generation of plasma
  • Efficacy (flank models)
  • Efficacy (orthotopic models)
  • Pharmacodynamic Endpoints
  • Imaging of cells with Xenogen System
  • Necropsy

 

Clinical Trial Support 

We isolate:

  • Plasma
  • Serum
  • Tumor Biopsy
  • Whole Blood (isolation of PBMC,
  • DNA, RNA, protein)
  • Bone Marrow (isolation of marrow
    cells)
  • Buccal Mucosa

 

Pharmacodynamic (PD) Endpoints 

  • in patient samples, tumor or surrogate tissues, preclinical samples
  • endpoint dependent on target (e.g., ELISA, flow cytometry, Western, unique assay)

Pleas acknowledge our core in your publications when appropriate in the format that follows:  “University of Maryland School of Medicine Center for Innovative Biomedical Resources, Translation Laboratory Shared Services Core – Baltimore, Maryland.

Leadership

Rena Lapidus, Ph.D., Director

Location and Hours of Operation

Hours Location

Monday - Friday    

8:30 am - 5:00 pm       

655 West Baltimore Street

Baltimore, MD  21201

Links and Resources

University of Maryland School of Medicine - Translational Core Laboratory

Biotek Synergy H1 Multimode Plate Reader

xCELLigence RTCA DP

 

Contacts

Name Role Phone Email Location
Rena Lapidus, PhD
Core Director
 
410-328-8092
 
RLapidus@som.umaryland.edu
 
9-037 BRB
 
Brandon Carter-Cooper
Lab Manager
 
410-706-2171
 
BCooper@som.umaryland.edu
 
7-010C BRB
 
Eun Yong Choi
In Vivo Manager
 
410-706-2171
 
echoi@som.umaryland.edu
 
7-010C BRB
 
Elizabeth Chang
Post-Doctoral Fellow
 
410-706-2171
 
echang@som.umaryland.edu
 
7-010C BRB
 
Julian Kang
Research Assistant
 
410-706-2171
 
jkang@som.umaryland.edu
 
7-010C BRB
 

Service list


Search available services: View: by category alphabetically
Clinical (5)
In Vitro (26)
In Vivo (17)
Plate Reader Fees (1)
xCELLigence Run Fees (3)


Members at Mid-Atlantic Shared Services Consortium

Core Name Primary Contact Email Phone Number/Ext
Biostatistics and Bioinformatics Shared Service

State-of-the-art statistical science, expert statistical analysis and sound statistical reporting

Susan Holt sholt@som.umaryland.edu (410) 706-8505
Confocal Microscopy Core

Equipment and expertise to assist investigators in applying advanced fluorescence microscopy techniques to their research.

Joseph Mauban, PhD jmauban@som.umaryland.edu 410-706-6170
Core for Translational Research in Imaging (CTRIM)

State-of-the-art technology for animal imaging at the molecular, cellular and systemic level. Services for in-vivo and ex-vivo cross-sectional and functional imaging using MRI, MR-g-FUS, PET, and CT.

Jiachen Zhuo jzhuo@umm.edu (410) 328-6443
Electron Microscopy Core Imaging Facility

‌‌‌‌‌Transmission Electron Microscope, Scanning Electron Microscope, SEM CryoTransfer & Chamber, High Pressure Freezer, Freeze Substitution System, Grid Plunger, CryoUltramicrotome, Gatan 626 cryo holder

Ru-Ching Hsia, PhD rhsia@umaryland.edu 410-7067992
Flow Cytometry Core

Sample acquisition on three analytical flow cytometers, expert data analysis, experimental planning and consultation and perform cell sorting on two high speed cell sorters.</p>

Karen Underwood, BS Flowcore@som.umaryland.edu 410-706-2173
Genomics Core Facility - Formerly Biopolymer Core Facility

DNA Sequencing, Cell Line Authentication, Gene Expression Microarray, Genotyping and more

Nicholas Ambulos, PhD nambulos@som.umaryland.edu 410-706-60665
Mass Spectrometry Center

Strives to accelerate discovery by providing investigators, on campus and beyond, access to cutting-edge technologies in mass spectrometry. Expetise from basic biology and medicine to technology development and translational research

Maureen A. Kane mkane@rx.umaryland.edu 410-706-5097
Pathology Biorepository Shared Service (PBSS)

Provides the collection of high quality banked patient samples while maintaining patient confidentiality. PBSS also provides pathology, histology, & histotechnology consultation services to assist principal investigators in the procurement, analyses, & clinicopathologic correlation of human tissue

Ashley Cellini, P.A. (ASCP) acellini@som.umaryland.edu 410-706-2174
Translational Laboratory Shared Service (UMGCC)

Pre-clinical and clinical experimental support to basic researchers and physicians. We work in areas across the entire spectrum: cell biology, in vitro, in vivo and human trials.

Rena Lapidus, PhD RLapidus@som.umaryland.edu 410-328-8092
Core Name Primary Contact Email Phone Number/Ext
Non-iLab, JHU SKCCC Core Facilities

Jeff Smith smithje@jhmi.edu
SKCCC IRAT Core (Image Response Assessment Team)

Michael Jacobs mjacobs8@jhmi.edu
SKCCC Immune Monitoring Core (formerly Human Immunology)

Christopher Thoburn cthoburn@jhmi.edu 410-955-8568
SKCCC Specimen Accessioning Core

Robin Stewart rstewa43@jhmi.edu 410 502-0674
SKCCC Analytical Pharmacology Core

Michelle Rudek Mrudek2@jhmi.edu 410-614-6321
SKCCC Animal Resources

Eric Davis edavisa@jhmi.edu 410-502-9544
SKCCC Cell Imaging Facility

Lillian Dasko-Vincent ldaskov1@jhmi.edu 410-614-4414
SKCCC Common Equipment Core

Eric Davis edavisa@jhmi.edu
SKCCC Cytogenetics Core Facility

Laura Morsberger lmorsber@jhmi.edu
SKCCC Experimental Irradiator Core

Esteban Velarde evelard2@jhmi.edu
SKCCC Flow Cytometry Core

Julie Nauroth jnaurot1@jhmi.edu 443-287-3952
SKCCC Microarray Core

Wayne Yu wyu8@jhmi.edu 410-502-7970
SKCCC Next Generation Sequencing Core

Lauren Ciotti nextgenseq@jhmi.edu 443-287-7341

University of Virginia

Core Name Primary Contact Email Phone Number/Ext
Advanced Microscopy Facility Stacey Criswell sjm7r@virginia.edu 434-924-2524
BioNMR Facility
Biomolecular Analysis Facility
Biorepository and Tissue Research Facility (BTRF) Craig Rumpel, MS crumpel@virginia.edu 434-982-6453
Flow Cytometry Core Facility FCCF Core Email fccf-staff2@virginia.edu
Genetically Engineered Murine Model (GEMM) Core
Merry X-Ray Leslie Yowell lfy8p@virginia.edu (434) 243-6051
Metabolomics Lipidomics Shared Resource Todd Fox tfox@virginia.edu 434-297-6516
MolMart & Tissue Culture Facility Linda Beggerly lkb7h@virginia.edu 434 924 9211
Molecular Electron Microscopy Core Kelly Dryden kdryden@virginia.edu 434.982.0525
Molecular Imaging Core Jack Roy jrr5a@virginia.edu (434) 243-9232
Office of Research Core Administration Alicia Johnson af7sb@virginia.edu 434-982-6565
SOM Clinical Research Unit (CRU)
Stem Cell Core Arazdordi Toumadje AT5KH@virginia.edu 434-297-5302
Surgical Skills Training Center Dana Davis Sikon dds4g@eservices.virginia.edu 434-924-6854