Welcome!
The University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) Translational Shared Resources(TLSR) offers pre-clinical and clinical experimental support to basic researchers and physicians in the UMGCC community. We work in areas across the entire spectrum: cell biology, in vitro, in vivo and human trials.
In Vitro Assays
Xcelligence
In Vivo Assays
Clinical Trial Support
We isolate:
Pharmacodynamic (PD) Endpoints
Rena Lapidus, Ph.D., Director
| Hours | Location |
|
Monday - Friday 8:30 am - 5:00 pm |
655 West Baltimore Street Baltimore, MD 21201 |
University of Maryland School of Medicine - Translational Laboratory Shared Resources (TLSR)
Biotek Synergy H1 Multimode Plate Reader
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Rena Lapidus, PhD |
Core Director
|
410-328-8092
|
RLapidus@som.umaryland.edu
|
BRB 7-043
|
| Brandon Carter-Cooper |
Lab Manager
|
410-706-2171
|
BCooper@som.umaryland.edu
|
BRB 7-010C
|
| Service list |
| ► Clinical (5) | |||
| Name | Description | Price | |
|---|---|---|---|
| Isolation of DNA from blood |
isolation of DNA (EZ1), label, freeze |
Inquire | |
| Isolation of PBMC from blood (funded trial) |
isolation of PBMC (ficoll), label, viably freeze |
Inquire | |
| Isolation of plasma (cfg, label, and freeze) | Inquire | ||
| Isolation of RNA from blood |
isolation of RNA (Ambion), label, freeze |
Inquire | |
| Isolation of serum (cfg, label and freeze) |
Isolation of serum, aliquot, label, freeze |
Inquire | |
| ► In Vitro (23) | |||
| Name | Description | Price | |
| AnnexinV (with flow cytometry) |
treat cells/3 time points/vehicle and two concentrations |
Inquire | |
| Biotek H1M Synergy Plate reader | Inquire | ||
| Biotek H1M Synergy Plate reader - kinetic | Inquire | ||
| Cell Cycle (with flow cytometry) |
treat cells/3 time points/vehicle and two concentrations |
Inquire | |
| Cell Line Aliquot Acquisition - cells have been STR validated and MAP tested | Inquire | ||
| Clonogenic with non adherent cell lines | Inquire | ||
| DNA Damage (Gamma-H2Ax Immunofluorescence) |
One assay including one compound/drug treatment (two concentrations), applicable controls and cell preparation, staining and imaging analysis. |
Inquire | |
| IC50 generation in cell lines - 3 pt |
3 point dose response |
Inquire | |
| IC50 generation in cell lines - 6-9 pt |
9 point dose response |
Inquire | |
| IC50 generation in normal bone marrow |
9 point dose repsonse |
Inquire | |
| IC50 generation in primary cells |
9 point dose response |
Inquire | |
| Mitopotential Red (with flow cytometry) |
treat cells/ 3 time points/vehicle and two concentrations |
Inquire | |
| mycoplasma - single | Inquire | ||
| Potentiation Study - |
Testing the combination of two compounds in vitro |
Inquire | |
| ROS generation |
fluorescent assay on plate reader |
Inquire | |
| Synergy - Chou Talalay Method |
Combination of two compounds in one cell line - IC50 of both individually and then combined in a fixed ratio IC pattern. Data entered into Calcusyn and Combination Index (CI) will be generatred |
Inquire | |
| Viability - non adherent cells |
4 point dose response
|
Inquire | |
| Viability - trypan blue - adherent cells |
4 point dose respnse |
Inquire | |
| Western Blot (no Tissue culture) |
up to three antibodies (not including antibody cost) |
Inquire | |
| xCelligence - adhesion | Inquire | ||
| xCelligence - invasion | Inquire | ||
| xCelligence - migration | Inquire | ||
| xCelligence - proliferation | Inquire | ||
| ► In Vivo (15) | |||
| Name | Description | Price | |
| Breast Primary Xenograft in MFP (PDX) Surgery | Inquire | ||
| Efficacy - Peritoneal Tumor Growth and Imaging with drug treatment | Inquire | ||
| Efficacy - flank (Syngeneic) | Inquire | ||
| Efficacy - flank (nude/NSG) |
less than 3x/week drug administration |
Inquire | |
| Efficacy - flank (nude/NSG) |
5x/week or daily drug administration |
Inquire | |
| Efficacy - flank with radiation (PI pays radiation expense) |
cost - one bolus radiation dose |
Inquire | |
| Efficacy -cells injected intravenous with imaging with drug treatment | Inquire | ||
| Estradiol Implantation | Inquire | ||
| Growth of Tumor and PI provides the cells (no cell culture by TLSS) | Inquire | ||
| Imaging Session (per hour) |
These will include mice that are imaged per hour ; fee includes cost of rental of Xenogen, luciferin and technician time. |
Inquire | |
| Maximum Tolerated Dose | Inquire | ||
| Mouse Bone Marrow Aspirate Isolation | Inquire | ||
| Mouse Injections |
Intravenous (IV), Intraperitoneal (IP), or Subcutaneous (SC). Price Listed is per mouse. |
Inquire | |
| Pharmacokinetic (PK) generation of plasma or serum (not tumor bearing) | Inquire | ||
| Tissue culture Services to grow cells for in vivo use | Inquire | ||
| ► Plate Reader Fees (1) | |||
| Name | Description | Price | |
| Standard Plate Read |
Charges per plate |
Inquire | |
| ► xCELLigence Run Fees (3) | |||
| Name | Description | Price | |
| Invasion Run | Inquire | ||
| Migration Run | Inquire | ||
| Proliferation Run | Inquire | ||
