Translational Laboratory Shared Resources (TLSR)

Please be sure to acknowledge us in your publications: University of Maryland School of Medicine’s & Greenebaum Comprehensive Cancer Center’s Translational Laboratory Shared Resources – Baltimore, Maryland. This publication was supported by funds through the Maryland Department of Health's Cigarette Restitution Fund Program. This publication was supported by funds through the Maryland Department of Health's Cigarette Restitution Fund Program and the National Cancer Institute - Cancer Center Support Grant (CCSG) - P30CA134274.

Overview of Services

The University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) Translational Shared Resources(TLSR) offers pre-clinical and clinical experimental support to basic researchers and physicians in the UMGCC community. We work in areas across the entire spectrum: cell biology, in vitro, in vivo and human trials.

In Vitro Assays

IC50 generation using MTS Proliferation Assay
Cell cycle (propidium iodide)
Viability (trypan blue exclusion)
Apoptosis/Annexin V
DNA Damage (Gamma-H2Ax Immunofluorescence)
Potentiation/Synergy
ROS (Microplate Reader Assay)
Western Analysis
Angiogenesis/Tube Formation
Mycoplasma testing

Xcelligence

Real time proliferation/invasion/migration

In Vivo Assays

IACUC approved umbrella protocol
Tolerability:
Tumor Growth
Pharmacokinetics: generation of plasma
Efficacy (flank models)
Efficacy (orthotopic models)
Pharmacodynamic Endpoints
Imaging of cells with Xenogen System
Necropsy

Clinical Trial Support

We isolate:

Plasma
Serum
Tumor Biopsy
Whole Blood (isolation of PBMC,
DNA, RNA, protein)
Bone Marrow (isolation of marrow
cells)
Buccal Mucosa

Pharmacodynamic (PD) Endpoints

in patient samples, tumor or surrogate tissues, preclinical samples
endpoint dependent on target (e.g., ELISA, flow cytometry, Western, unique assay)

Leadership

Rena Lapidus, Ph.D., Director

Location and Hours of Operation

Hours

Location

Monday - Friday

8:30 am - 5:00 pm

655 West Baltimore Street

Baltimore, MD 21201

Links and Resources

University of Maryland School of Medicine - Translational Laboratory Shared Resources (TLSR)

Biotek Synergy H1 Multimode Plate Reader

xCELLigence RTCA DP

Contacts

Name	Role	Phone	Email	Location
Rena Lapidus, PhD	Core Director	410-328-8092	RLapidus@som.umaryland.edu	BRB 7-043
Brandon Carter-Cooper	Lab Manager	410-706-2171	BCooper@som.umaryland.edu	BRB 7-010C

Service list

Name	Description	Price
AnnexinV (with flow cytometry)	treat cells/3 time points/vehicle and two concentrations	Inquire
Biotek H1M Synergy Plate reader		Inquire
Biotek H1M Synergy Plate reader - kinetic		Inquire
Breast Primary Xenograft in MFP (PDX) Surgery		Inquire
Clonogenic with non adherent cell lines		Inquire
CRISPR Knock Out (Difficult)		Inquire
CRISPR Knock Out (Easy)		Inquire
CRISPR SNP (Difficult)		Inquire
CRISPR SNP (Easy)		Inquire
CY26 In Vitro Transduction (Easy)		Inquire
DNA Damage (Gamma-H2Ax Immunofluorescence)	One assay including one compound/drug treatment (two concentrations), applicable controls and cell preparation, staining and imaging analysis.	Inquire
Efficacy - Peritoneal Tumor Growth and Imaging with drug treatment		Inquire
Efficacy - flank (Syngeneic)		Inquire
Efficacy - flank (nude/NSG)	5x/week	Inquire
Efficacy - flank (nude/NSG)	less than 3x/week drug administration	Inquire
Efficacy - flank with radiation (PI pays radiation expense)	cost - one bolus radiation dose	Inquire
Efficacy -cells injected intravenous with imaging with drug treatment		Inquire
Estradiol Implantation		Inquire
Fresh or Frozen Tissue Specimen		Inquire
Growth of Tumor and PI provides the cells (no cell culture by TLSS)		Inquire
Imaging Session (per hour)	These will include mice that are imaged per hour ; fee includes cost of rental of Xenogen, luciferin and technician time.	Inquire
In Vitro Transduction (Difficult)		Inquire
Invasion Run		Inquire
Isolation of plasma/serum (cfg, label and freeze)	Isolation of serum, aliquot, label, freeze	Inquire
Maximum Tolerated Dose with expansive necropsy		Inquire
Migration Run		Inquire
Mouse Bone Marrow Aspirate Isolation		Inquire
Mouse Injections	Intravenous (IV), Intraperitoneal (IP), or Subcutaneous (SC). Price Listed is per mouse.	Inquire
Pharmacokinetic (PK) generation of plasma or serum (not tumor bearing)		Inquire
Proliferation Run		Inquire
Standard Plate Read	Charges per plate	Inquire
Synergy - Chou Talalay Method	Combination of two compounds in one cell line - IC50 of both individually and then combined in a fixed ratio IC pattern. Data entered into Calcusyn and Combination Index (CI) will be generatred	Inquire
Tissue culture Services to grow cells for in vivo use		Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► CY26 CRISPR (4)
CRISPR Knock Out (Difficult)		Inquire
CRISPR Knock Out (Easy)		Inquire
CRISPR SNP (Difficult)		Inquire
CRISPR SNP (Easy)		Inquire
▼ ► CY26 Clinical (2)
Name	Description	Price
Fresh or Frozen Tissue Specimen		Inquire
Isolation of plasma/serum (cfg, label and freeze)	Isolation of serum, aliquot, label, freeze	Inquire
▼ ► CY26 In Vitro (4)
Name	Description	Price
AnnexinV (with flow cytometry)	treat cells/3 time points/vehicle and two concentrations	Inquire
Clonogenic with non adherent cell lines		Inquire
CY26 In Vitro Transduction (Easy)		Inquire
In Vitro Transduction (Difficult)		Inquire
▼ ► In Vitro (4)
Name	Description	Price
Biotek H1M Synergy Plate reader		Inquire
Biotek H1M Synergy Plate reader - kinetic		Inquire
DNA Damage (Gamma-H2Ax Immunofluorescence)	One assay including one compound/drug treatment (two concentrations), applicable controls and cell preparation, staining and imaging analysis.	Inquire
Synergy - Chou Talalay Method	Combination of two compounds in one cell line - IC50 of both individually and then combined in a fixed ratio IC pattern. Data entered into Calcusyn and Combination Index (CI) will be generatred	Inquire
▼ ► In Vivo (15)
Name	Description	Price
Breast Primary Xenograft in MFP (PDX) Surgery		Inquire
Efficacy - Peritoneal Tumor Growth and Imaging with drug treatment		Inquire
Efficacy - flank (Syngeneic)		Inquire
Efficacy - flank (nude/NSG)	less than 3x/week drug administration	Inquire
Efficacy - flank (nude/NSG)	5x/week	Inquire
Efficacy - flank with radiation (PI pays radiation expense)	cost - one bolus radiation dose	Inquire
Efficacy -cells injected intravenous with imaging with drug treatment		Inquire
Estradiol Implantation		Inquire
Growth of Tumor and PI provides the cells (no cell culture by TLSS)		Inquire
Imaging Session (per hour)	These will include mice that are imaged per hour ; fee includes cost of rental of Xenogen, luciferin and technician time.	Inquire
Maximum Tolerated Dose with expansive necropsy		Inquire
Mouse Bone Marrow Aspirate Isolation		Inquire
Mouse Injections	Intravenous (IV), Intraperitoneal (IP), or Subcutaneous (SC). Price Listed is per mouse.	Inquire
Pharmacokinetic (PK) generation of plasma or serum (not tumor bearing)		Inquire
Tissue culture Services to grow cells for in vivo use		Inquire
▼ ► Plate Reader Fees (1)
Name	Description	Price
Standard Plate Read	Charges per plate	Inquire
▼ ► xCELLigence Run Fees (3)
Name	Description	Price
Invasion Run		Inquire
Migration Run		Inquire
Proliferation Run		Inquire