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ALERT ATTENTION ⚠️
In Observance of the holidays, Agilent CrossLab/ iLab Operations Software Support Help Desk will be closed during U.S. hours on Wednesday January 1st, 2025. We will resume regular U.S. support hours on Thursday January 2nd, 2025. For urgent matters, please add "Urgent" to the ticket/ email subject or press "1", when prompted, to escalate a call on the iLab Support phone and we will prioritize those requests first.
The University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) Translational Shared Resources(TLSR) offers pre-clinical and clinical experimental support to basic researchers and physicians in the UMGCC community. We work in areas across the entire spectrum: cell biology, in vitro, in vivo and human trials.
In Vitro Assays
Xcelligence
In Vivo Assays
Clinical Trial Support
We isolate:
Pharmacodynamic (PD) Endpoints
Rena Lapidus, Ph.D., Director
| Hours | Location |
|
Monday - Friday 8:30 am - 5:00 pm |
655 West Baltimore Street Baltimore, MD 21201 |
University of Maryland School of Medicine - Translational Laboratory Shared Resources (TLSR)
Biotek Synergy H1 Multimode Plate Reader
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Rena Lapidus, PhD |
Core Director
|
410-328-8092
|
RLapidus@som.umaryland.edu
|
BRB 7-043
|
| Brandon Carter-Cooper |
Lab Manager
|
410-706-2171
|
BCooper@som.umaryland.edu
|
BRB 7-010C
|
| Service list |
| ► Clinical (5) | |||
| Name | Description | Price | |
|---|---|---|---|
| Isolation of DNA from blood |
isolation of DNA (EZ1), label, freeze |
Inquire | |
| Isolation of PBMC from blood (funded trial) |
isolation of PBMC (ficoll), label, viably freeze |
Inquire | |
| Isolation of plasma (cfg, label, and freeze) | Inquire | ||
| Isolation of RNA from blood |
isolation of RNA (Ambion), label, freeze |
Inquire | |
| Isolation of serum (cfg, label and freeze) |
Isolation of serum, aliquot, label, freeze |
Inquire | |
| ► In Vitro (23) | |||
| Name | Description | Price | |
| AnnexinV (with flow cytometry) |
treat cells/3 time points/vehicle and two concentrations |
Inquire | |
| Biotek H1M Synergy Plate reader | Inquire | ||
| Biotek H1M Synergy Plate reader - kinetic | Inquire | ||
| Cell Cycle (with flow cytometry) |
treat cells/3 time points/vehicle and two concentrations |
Inquire | |
| Cell Line Aliquot Acquisition - cells have been STR validated and MAP tested | Inquire | ||
| Clonogenic with non adherent cell lines | Inquire | ||
| DNA Damage (Gamma-H2Ax Immunofluorescence) |
One assay including one compound/drug treatment (two concentrations), applicable controls and cell preparation, staining and imaging analysis. |
Inquire | |
| IC50 generation in cell lines - 3 pt |
3 point dose response |
Inquire | |
| IC50 generation in cell lines - 6-9 pt |
9 point dose response |
Inquire | |
| IC50 generation in normal bone marrow |
9 point dose repsonse |
Inquire | |
| IC50 generation in primary cells |
9 point dose response |
Inquire | |
| Mitopotential Red (with flow cytometry) |
treat cells/ 3 time points/vehicle and two concentrations |
Inquire | |
| mycoplasma - single | Inquire | ||
| Potentiation Study - |
Testing the combination of two compounds in vitro |
Inquire | |
| ROS generation |
fluorescent assay on plate reader |
Inquire | |
| Synergy - Chou Talalay Method |
Combination of two compounds in one cell line - IC50 of both individually and then combined in a fixed ratio IC pattern. Data entered into Calcusyn and Combination Index (CI) will be generatred |
Inquire | |
| Viability - non adherent cells |
4 point dose response
|
Inquire | |
| Viability - trypan blue - adherent cells |
4 point dose respnse |
Inquire | |
| Western Blot (no Tissue culture) |
up to three antibodies (not including antibody cost) |
Inquire | |
| xCelligence - adhesion | Inquire | ||
| xCelligence - invasion | Inquire | ||
| xCelligence - migration | Inquire | ||
| xCelligence - proliferation | Inquire | ||
| ► In Vivo (15) | |||
| Name | Description | Price | |
| Breast Primary Xenograft in MFP (PDX) Surgery | Inquire | ||
| Efficacy - Peritoneal Tumor Growth and Imaging with drug treatment | Inquire | ||
| Efficacy - flank (Syngeneic) | Inquire | ||
| Efficacy - flank (nude/NSG) |
less than 3x/week drug administration |
Inquire | |
| Efficacy - flank (nude/NSG) |
5x/week or daily drug administration |
Inquire | |
| Efficacy - flank with radiation (PI pays radiation expense) |
cost - one bolus radiation dose |
Inquire | |
| Efficacy -cells injected intravenous with imaging with drug treatment | Inquire | ||
| Estradiol Implantation | Inquire | ||
| Growth of Tumor and PI provides the cells (no cell culture by TLSS) | Inquire | ||
| Imaging Session (per hour) |
These will include mice that are imaged per hour ; fee includes cost of rental of Xenogen, luciferin and technician time. |
Inquire | |
| Maximum Tolerated Dose | Inquire | ||
| Mouse Bone Marrow Aspirate Isolation | Inquire | ||
| Mouse Injections |
Intravenous (IV), Intraperitoneal (IP), or Subcutaneous (SC). Price Listed is per mouse. |
Inquire | |
| Pharmacokinetic (PK) generation of plasma or serum (not tumor bearing) | Inquire | ||
| Tissue culture Services to grow cells for in vivo use | Inquire | ||
| ► Plate Reader Fees (1) | |||
| Name | Description | Price | |
| Standard Plate Read |
Charges per plate |
Inquire | |
| ► xCELLigence Run Fees (3) | |||
| Name | Description | Price | |
| Invasion Run | Inquire | ||
| Migration Run | Inquire | ||
| Proliferation Run | Inquire | ||
