Welcome!
The mission of the Biopolymer/Genomics Core Facility is to enable and foster institutional science by providing the expertise, state-of-the-art resources, and the training necessary to promote cutting edge genomic research.
The Facility and the personnel that staff it, are committed to maintaining technologically advanced instrumentation and providing an educational environment to instruct faculty, staff, fellows, and students on the latest technologies and how they can positively impact on their research.
Our staff is available to share their extensive knowledge and expertise in order to successfully support the research being conducted within the institution.
The Biopolymer/Genomics Core Facility maintains a number of instruments that are available for investigators to use once they have been properly trained. Investigators are responsible for any consumables necessary, and user fees charged are necessary to cover maintenance costs.
Pleas acknowledge our core in your publications when appropriate in the format that follows: “University of Maryland School of Medicine Center for Innovative Biomedical Resources, Genomics Core – Baltimore, Maryland.
Nicholas Ambulos, PhD, Director
nambulos@som.umaryland.edu
| Hours | Location |
|
Monday - Friday 7 am - 5:30 pm |
655 W Baltimore Street Bressler Building, 7th Floor Baltimore, MD 21201 |
University of Maryland, School of Medicine - Biopolymer/Genomics Core Facility
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Nicholas Ambulos, PhD |
Core Director
|
410-706-60665
|
nambulos@som.umaryland.edu
|
Bressler Building 7-010-D
|
| Jing Yin |
Research Manager
|
410-706-8553
|
jyin@umaryland.edu
|
Bressler Building 7-010-D
|
| Li Tang |
Research Specialist
|
410-706-8553
|
Ltang@som.umaryland.edu
|
Bressler Building 7-010-D
|
| Kevin Rossomando |
Research Assitant
|
410-706-3339
|
Biopolymer@som.umaryland.edu
|
Bressler Building 7-010-D
|
| Prices |
| ► Cell Line Authentication (2) | |||
| Name | Description | Price | |
|---|---|---|---|
| Human Cell Line Authentication STR typing PLUS DNA Extraction from cell pellet | Inquire | ||
| Human Cell Line Authentication STR Typing ONLY (allele table returned) | Inquire | ||
| ► DNA Sequencing (5) | |||
| Name | Description | Price | |
| Denville ThermoGrid PCR Plates, 96 well Mini Skirt plate, package of 10 |
Can be ordered as a stand alone purchase. Core staff may need to add to full plate DNA Sequencing orders |
Inquire | |
| DNA Sequencing - Full reactions ($400 per 96 well tray) | Inquire | ||
| DNA Sequencing - Full reactions (we attach dye) | Inquire | ||
| DNA Sequencing - Ready to run ($40 per Full 96 well trays) | Inquire | ||
| DNA Sequencing - Single Runs (Ready to run, dye attached) | Inquire | ||
| ► DNA and RNA Extraction (2) | |||
| Name | Description | Price | |
| DNA Extraction | Inquire | ||
| RNA Extraction | Inquire | ||
| ► Gene Expression Microarray (7) | |||
| Name | Description | Price | |
| ARRAY COSTS - Gene Expression Microarray | Inquire | ||
| Full service , Affymetrix Gene Expression, Exon Array - Starting from total RNA | Inquire | ||
| Full Service, Affymetrix 3' IVT Array, Exon Array - Starting from total RNA | Inquire | ||
| Full service, Affymetrix Gene Expression, Gene ST Array - Starting from total RNA | Inquire | ||
| Full service, Affymetrix microRNA Expression Arrays - Starting from total RNA | Inquire | ||
| Full service, Affymetrix XTA Array - Starting from total RNA | Inquire | ||
| Gene Expression Microarray Rehybridization of old samples using new arrays | Inquire | ||
| ► Genotyping (6) | |||
| Name | Description | Price | |
| 7900 Real-Time PCR runs | Inquire | ||
| ARRAY COSTS -Genotyping | Inquire | ||
| Full service Taqman Genotyping (96 well) - starting from genomic DNA | Inquire | ||
| Full service, Affymetrix SNP 6.0 Array - starting from genomic DNA | Inquire | ||
| Genotyping Hybridization only - Starting from labeled, fragmented material | Inquire | ||
| Genotyping Rehybridization of old samples using new arrays | Inquire | ||
| ► Miscellaneous Custom Services (8) | |||
| Name | Description | Price | |
| Agilent Bioanalyzer RNA 6000 Nano Kit |
Up to 12 per chip |
Inquire | |
| Agilent Bioanalyzer RNA 6000 Pico Kit |
Up to 11 per chip |
Inquire | |
| Agilent DNA 1000 Kit |
Up to 12 per chip |
Inquire | |
| Agilent DNA 12000 Kit |
Up to 12 per chip |
Inquire | |
| Agilent High Sensitivity DNA Kit |
Upto 11 per chip |
Inquire | |
| PCR Runs | Inquire | ||
| Purification | Inquire | ||
| rtPCR Runs | Inquire | ||
| ► Protein Analysis Services (5) | |||
| Name | Description | Price | |
| HPLC/DAD Instrument Time |
High Pressure Liquid Chromatography with photo diode array detection (200nm to 800nm). |
Inquire | |
| LC/MS High Resolution and Mass Accuracy (Orbitrap) |
Orbitrap Elite instrument time. |
Internal
$80.00
/hr
External Academic Non-profit $100.00 /hr External For Profit $150.00 /hr |
|
| LC/MS Triple Quad Instrument Time |
Liquid Chromatography Tandem Mass spectrometry using a triple quadrupole mass spectrometer, e.g. SRM assays. |
Inquire | |
| Quantitative Protein Profiling |
Compare the relative concentrations of hundreds to thousands of proteins between two or more samples. Includes Trypsin digestion, LC-MS/MS, database search, relative quantification using the MaxQuant/Perseus pipeline. |
Inquire | |
| Simple Protein Identification |
Protein ID from gel bands or in solution where the protein amount is high and requires minimal processing. Can be used in purification workflows for confirming the protein identity in fractions, after cleavage from affinity tag etc. |
Inquire | |
