The Center for Biomolecular Therapeutics (CBT)

Complimentary consultation meeting with PEB and SBN Section Leaders to discuss final application.

CryoTEM grids are prepared for user supplied specimen.

This service generally includes collected small to medium size datasets (up to 24 hours of data collection) on pre-made cryoTEM grids followed by standard data processing and evaluation of the sample quality and feasibility of obtaining atomic resolution structure by cryoEM (up to generation of 2D classes and, if possible, initial 3D strutural models in form of electron density).

Final cost is variable and depends on length of construct and fusion tags/proteins.

CryoTEM grids need to be "clipped" in order to be compatible with the autoloader system of TFS-FEI electron microscopes.  This service is provided when grids themselves are already prepared by the user and stored at the facility.  Includes clipping supplies (C-clip/C-ring, $20 per grid when purchased from the vendor) 

Large scale protein expression will be done once protein SEL are known. The cost of this phase will vary depending on the isotopic labeling used.

Purified protein will be provided with >95% purity by SDS-PAGE and Mass Spec.

• Accurate protein concentration determination.
• Labeled vial and datasheet with protein concentration, final molecular weight of purified protein, complete buffer components, pH, and volume.
• Detailed list of sample conditions and NMR parameters used for collecting the data

Expression vectors will be designed based on available literature. Soluble expression levels (SEL) in E. coli will be tested and verified using SDS-PAGE. It may also be determined, at this step, that refolding of the protein from inclusion bodies may be necessary.  If purification from inclusion bodies were needed, additional studies would be necessary, which are not included in this estimate.

CryoTEM grids are prepared for user supplied specimen.

This service generally includes collected small to medium size datasets (up to 24 hours of data collection) on pre-made cryoTEM grids followed by standard data processing and evaluation of the sample quality and feasibility of obtaining atomic resolution structure by cryoEM (up to generation of 2D classes and, if possible, initial 3D strutural models in form of electron density).

CryoTEM grids need to be "clipped" in order to be compatible with the autoloader system of TFS-FEI electron microscopes.  This service is provided when grids themselves are already prepared by the user and stored at the facility.  Includes clipping supplies (C-clip/C-ring, $20 per grid when purchased from the vendor) 

Complimentary consultation meeting with PEB and SBN Section Leaders to discuss final application.

Final cost is variable and depends on length of construct and fusion tags/proteins.

Large scale protein expression will be done once protein SEL are known. The cost of this phase will vary depending on the isotopic labeling used.

Purified protein will be provided with >95% purity by SDS-PAGE and Mass Spec.

• Accurate protein concentration determination.
• Labeled vial and datasheet with protein concentration, final molecular weight of purified protein, complete buffer components, pH, and volume.
• Detailed list of sample conditions and NMR parameters used for collecting the data

Expression vectors will be designed based on available literature. Soluble expression levels (SEL) in E. coli will be tested and verified using SDS-PAGE. It may also be determined, at this step, that refolding of the protein from inclusion bodies may be necessary.  If purification from inclusion bodies were needed, additional studies would be necessary, which are not included in this estimate.